fadd sc 5559 Search Results


fadd  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology fadd
HCD activates the death receptor‐mediated apoptotic signaling pathway in SCC cells. (A) SCC25 cells were incubated with HCD (IC 50 ) for 24, 48 and 72 h, after which the gene expression levels of death receptor‐associated apoptosis pathway were determined by RT‐PCR analysis. Treating SCC25 cells with (+) or without HCD (IC 50 ) for 72 h, and the protein expressions of TNF‐α, TNF‐R1, TNF‐R2, FasL, <t>Fas,</t> <t>TRADD</t> and <t>FADD</t> were assessed by western blotting (B), immunofluorescence microscopy (C) and flow cytometry analysis (D). β‐Actin was measured as the quality control. The blot was cut after transfer to allow separate probing with anti‐β‐Actin and anti‐target protein antibodies. The splice is indicated by a black line. Histograms represent statistical analysis of the relative expression levels. The nuclei were visualized by staining DNA with Hoechst 33342 (blue) and using a fluorescent microscope (200×). Arrowheads indicate cells feature. Nuclei were visualized by staining DNA with Hoechst 33342 (blue) and certain protein expressions were stained with FITC (green) using a fluorescent microscope (200×). Arrowheads indicate apoptotic cells expressing a certain protein. The percentage as defined by fluorescent stained protein expression levels of cells and compared to control cells by flow cytometry. All data are presented as mean ± SD from three independent biological replicates ( n = 3 per condition). Statistical significance was determined using appropriate tests, with * p < 0.01 and p < 0.05 indicating significant differences compared to DMSO (vehicle control)‐treated cells.
Fadd, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fadd+sc+5559/pmc12774572-80-82-83?v=Santa+Cruz+Biotechnology
Average 93 stars, based on 1 article reviews
fadd - by Bioz Stars, 2026-08
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anti fadd  (Santa Cruz Biotechnology)
95
Santa Cruz Biotechnology anti fadd
HCD activates the death receptor‐mediated apoptotic signaling pathway in SCC cells. (A) SCC25 cells were incubated with HCD (IC 50 ) for 24, 48 and 72 h, after which the gene expression levels of death receptor‐associated apoptosis pathway were determined by RT‐PCR analysis. Treating SCC25 cells with (+) or without HCD (IC 50 ) for 72 h, and the protein expressions of TNF‐α, TNF‐R1, TNF‐R2, FasL, <t>Fas,</t> <t>TRADD</t> and <t>FADD</t> were assessed by western blotting (B), immunofluorescence microscopy (C) and flow cytometry analysis (D). β‐Actin was measured as the quality control. The blot was cut after transfer to allow separate probing with anti‐β‐Actin and anti‐target protein antibodies. The splice is indicated by a black line. Histograms represent statistical analysis of the relative expression levels. The nuclei were visualized by staining DNA with Hoechst 33342 (blue) and using a fluorescent microscope (200×). Arrowheads indicate cells feature. Nuclei were visualized by staining DNA with Hoechst 33342 (blue) and certain protein expressions were stained with FITC (green) using a fluorescent microscope (200×). Arrowheads indicate apoptotic cells expressing a certain protein. The percentage as defined by fluorescent stained protein expression levels of cells and compared to control cells by flow cytometry. All data are presented as mean ± SD from three independent biological replicates ( n = 3 per condition). Statistical significance was determined using appropriate tests, with * p < 0.01 and p < 0.05 indicating significant differences compared to DMSO (vehicle control)‐treated cells.
Anti Fadd, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fadd+sc+5559/pmc03176567-242-27-33?v=Santa+Cruz+Biotechnology
Average 95 stars, based on 1 article reviews
anti fadd - by Bioz Stars, 2026-08
95/100 stars
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fadd  (Becton Dickinson)
90
Becton Dickinson fadd
HCD activates the death receptor‐mediated apoptotic signaling pathway in SCC cells. (A) SCC25 cells were incubated with HCD (IC 50 ) for 24, 48 and 72 h, after which the gene expression levels of death receptor‐associated apoptosis pathway were determined by RT‐PCR analysis. Treating SCC25 cells with (+) or without HCD (IC 50 ) for 72 h, and the protein expressions of TNF‐α, TNF‐R1, TNF‐R2, FasL, <t>Fas,</t> <t>TRADD</t> and <t>FADD</t> were assessed by western blotting (B), immunofluorescence microscopy (C) and flow cytometry analysis (D). β‐Actin was measured as the quality control. The blot was cut after transfer to allow separate probing with anti‐β‐Actin and anti‐target protein antibodies. The splice is indicated by a black line. Histograms represent statistical analysis of the relative expression levels. The nuclei were visualized by staining DNA with Hoechst 33342 (blue) and using a fluorescent microscope (200×). Arrowheads indicate cells feature. Nuclei were visualized by staining DNA with Hoechst 33342 (blue) and certain protein expressions were stained with FITC (green) using a fluorescent microscope (200×). Arrowheads indicate apoptotic cells expressing a certain protein. The percentage as defined by fluorescent stained protein expression levels of cells and compared to control cells by flow cytometry. All data are presented as mean ± SD from three independent biological replicates ( n = 3 per condition). Statistical significance was determined using appropriate tests, with * p < 0.01 and p < 0.05 indicating significant differences compared to DMSO (vehicle control)‐treated cells.
Fadd, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fadd+sc+5559/pm22864571-284-7-9?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
fadd - by Bioz Stars, 2026-08
90/100 stars
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Image Search Results


HCD activates the death receptor‐mediated apoptotic signaling pathway in SCC cells. (A) SCC25 cells were incubated with HCD (IC 50 ) for 24, 48 and 72 h, after which the gene expression levels of death receptor‐associated apoptosis pathway were determined by RT‐PCR analysis. Treating SCC25 cells with (+) or without HCD (IC 50 ) for 72 h, and the protein expressions of TNF‐α, TNF‐R1, TNF‐R2, FasL, Fas, TRADD and FADD were assessed by western blotting (B), immunofluorescence microscopy (C) and flow cytometry analysis (D). β‐Actin was measured as the quality control. The blot was cut after transfer to allow separate probing with anti‐β‐Actin and anti‐target protein antibodies. The splice is indicated by a black line. Histograms represent statistical analysis of the relative expression levels. The nuclei were visualized by staining DNA with Hoechst 33342 (blue) and using a fluorescent microscope (200×). Arrowheads indicate cells feature. Nuclei were visualized by staining DNA with Hoechst 33342 (blue) and certain protein expressions were stained with FITC (green) using a fluorescent microscope (200×). Arrowheads indicate apoptotic cells expressing a certain protein. The percentage as defined by fluorescent stained protein expression levels of cells and compared to control cells by flow cytometry. All data are presented as mean ± SD from three independent biological replicates ( n = 3 per condition). Statistical significance was determined using appropriate tests, with * p < 0.01 and p < 0.05 indicating significant differences compared to DMSO (vehicle control)‐treated cells.

Journal: Journal of Oral Pathology & Medicine

Article Title: The PI3K Inhibitor HCD Promotes Caspase Activation in Head and Neck Squamous Cells by Upregulating the Extrinsic and Intrinsic Apoptosis Pathways

doi: 10.1111/jop.70060

Figure Lengend Snippet: HCD activates the death receptor‐mediated apoptotic signaling pathway in SCC cells. (A) SCC25 cells were incubated with HCD (IC 50 ) for 24, 48 and 72 h, after which the gene expression levels of death receptor‐associated apoptosis pathway were determined by RT‐PCR analysis. Treating SCC25 cells with (+) or without HCD (IC 50 ) for 72 h, and the protein expressions of TNF‐α, TNF‐R1, TNF‐R2, FasL, Fas, TRADD and FADD were assessed by western blotting (B), immunofluorescence microscopy (C) and flow cytometry analysis (D). β‐Actin was measured as the quality control. The blot was cut after transfer to allow separate probing with anti‐β‐Actin and anti‐target protein antibodies. The splice is indicated by a black line. Histograms represent statistical analysis of the relative expression levels. The nuclei were visualized by staining DNA with Hoechst 33342 (blue) and using a fluorescent microscope (200×). Arrowheads indicate cells feature. Nuclei were visualized by staining DNA with Hoechst 33342 (blue) and certain protein expressions were stained with FITC (green) using a fluorescent microscope (200×). Arrowheads indicate apoptotic cells expressing a certain protein. The percentage as defined by fluorescent stained protein expression levels of cells and compared to control cells by flow cytometry. All data are presented as mean ± SD from three independent biological replicates ( n = 3 per condition). Statistical significance was determined using appropriate tests, with * p < 0.01 and p < 0.05 indicating significant differences compared to DMSO (vehicle control)‐treated cells.

Article Snippet: The cells were then probed with specific antibodies against cyto. c (Santa Cruz Biotechnology, sc‐13560, RRID: AB_627383, 1:200), Bax (Santa Cruz Biotechnology, sc‐20067, RRID: AB_626726, 1:200), Bcl‐2 (Santa Cruz Biotechnology, sc‐7382, RRID: AB_626736, 1:200), TNF‐α (Santa Cruz Biotechnology, sc‐33639, RRID: AB_628371, 1:200), TNF‐R1 (Santa Cruz Biotechnology, sc‐1067, RRID: AB_632519, 1:200), TNF‐R2 (Santa Cruz Biotechnology, sc‐1074, RRID: AB_2206681, 1:200), FasL (Santa Cruz Biotechnology, sc‐834, RRID: AB_2100640, 1:200), Fas (Santa Cruz Biotechnology, sc‐8009, RRID: AB_627218, 1:200), TRADD (Santa Cruz Biotechnology, sc‐46653, RRID: AB_2209061, 1:200), FADD (Santa Cruz Biotechnology, sc‐5559, RRID: AB_2209061, 1:200), Caspase‐9 (Santa Cruz Biotechnology, sc‐17784, RRID: AB_673489), cleaved Caspase‐8 (Cell signaling technology, #9496, RRID: AB_561381, 1:1000), cleaved Caspase‐3 (Cell signaling technology, #9661, RRID: AB_2341188, 1:1000), and β‐actin (Santa Cruz Biotechnology, sc‐47 778, RRID: AB_2714189, 1:500) as outlined in a previously described protocol.

Techniques: Incubation, Gene Expression, Reverse Transcription Polymerase Chain Reaction, Western Blot, Immunofluorescence, Microscopy, Flow Cytometry, Control, Expressing, Staining